The pLW01 Expression Vector
The expression vector pLW01, is a small, mulitcopy plasmid developed by Dr. Lucy Waskell at the University of Michigan. The pLW01 expression vector [4] has a standard T7 promoter site and uses ampicillin resistance for selection. This vector has been quite effective in expressing single transit membrane proteins, like native mammalian cytochrome P450s [1-3] and cytochrome b5 [4], with yields as high as 50 mg of purified protein per liter of culture. Here is information about the full-length sequence and the MCS of the pLW01 vector:
Download plasmid sequence: pLW01_plasmid.pdf
Download sequence of the multicloning site: pLW01_MCS.pdf
Samples of the pLW01 expression vector for academic research can be obtained through the CSBMP directly; commercial users should contact Dr. Waskell directly. Publication of research involving the pLW01 expression vector should attribute the source of the vector as being from Dr. Lucy Waskell, University of Michigan , but through auspices of the CSBMP.
1. Bridges A, Gruenke L, Chang YT, Vakser IA, Loew G, Waskell L: Identification of the binding site on cytochrome P450 2B4 for cytochrome b5 and cytochrome P450 reductase. J Biol Chem 1998, 273:17036-17049.
2. Cheng D, Kelley RW, Cawley GF, Backes WL: High-level expression of recombinant rabbit cytochrome P450 2E1 in Escherichia coli C41 and its purification. Protein Expr Purif 2004, 33:66-71.
3. Saribas AS, Gruenke L, Waskell L: Overexpression and purification of the membrane-bound cytochrome P450 2B4. Protein Expr Purif 2001, 21:303-309.
4. Mulrooney SB, Waskell L: High-level expression in Escherichia coli and purification of the membrane-bound form of cytochrome b(5). Protein Expr Purif 2000, 19:173-178.